Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices.

ABSTRACT: Extracellular matrix is a key component of many products in regenerative medicine. Multiple regenerative medicine products currently in the clinic are comprised of human or xenogeneic extracellular matrix. In addition, whole-organ regeneration exploits decellularized native organs as scaffolds for organotypic cell culture. However, precise understanding of the constituents of such extracellular matrix-based implants and scaffolds has sorely lagged behind their use. We present here an advanced protein extraction method using known quantities of proteotypic ¹³C-labeled peptides to quantify matrix proteins in native and decellularized lung tissues. Using quantitative proteomics that produce picomole-level measurements of a large number of matrix proteins, we show that a mild decell

SUBMITTER: Calle EA 

PROVIDER: S-EPMC5451113 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature