Bispecific antibody targets multiple Pseudomonas aeruginosa evasion mechanisms in the lung vasculature.

ABSTRACT: Pseudomonas aeruginosa is a major cause of severe infections that lead to bacteremia and high patient mortality. P. aeruginosa has evolved numerous evasion and subversion mechanisms that work in concert to overcome immune recognition and effector functions in hospitalized and immunosuppressed individuals. Here, we have used multilaser spinning-disk intravital microscopy to monitor the blood-borne stage in a murine bacteremic model of P. aeruginosa infection. P. aeruginosa adhered avidly to lung vasculature, where patrolling neutrophils and other immune cells were virtually blind to the pathogen's presence. This cloaking phenomenon was attributed to expression of Psl exopolysaccharide. Although an anti-Psl mAb activated complement and enhanced neutrophil recognition of P. aeruginosa, neutro

SUBMITTER: Thanabalasuriar A 

PROVIDER: S-EPMC5451222 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature