The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain diet.

ABSTRACT:

Background

The newly proposed methanogenic order 'Methanomassiliicoccales' is the second largest archaeal population in the rumen, second only to the Methanobrevibacter population. However, information is limited regarding the community of this new order in the rumen.

Methods

This study used real-time PCR and 454 pyrosequencing to explore the abundance and community composition of Methanomassiliicoccales in the rumen of Chinese goats fed a hay (0% grain, n = 5) or a high grain (65% grain, n = 5) diet.

Results

Real-time PCR analysis showed that the relative abundance of Methanomassiliicoccales (% of total archaea) in the goat rumen was significantly lower in the high-grain-diet group (0.5% ± 0.2%) than that in the hay-diet grou

SUBMITTER: Jin W 

PROVIDER: S-EPMC5452365 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature