Single-cell mRNA profiling reveals transcriptional heterogeneity among pancreatic circulating tumour cells.

ABSTRACT:

Background

Single-cell mRNA profiling of circulating tumour cells may contribute to a better understanding of the biology of these cells and their role in the metastatic process. In addition, such analyses may reveal new knowledge about the mechanisms underlying chemotherapy resistance and tumour progression in patients with cancer.

Methods

Single circulating tumour cells were isolated from patients with locally advanced or metastatic pancreatic cancer with immuno-magnetic depletion and immuno-fluorescence microscopy. mRNA expression was analysed with single-cell multiplex RT-qPCR. Hierarchical clustering and principal component analysis were performed to identify expression patterns.

Results

Circulating tumour cells were detected in 33 of 56 (59%) examined blood samp

SUBMITTER: Lapin M 

PROVIDER: S-EPMC5452374 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature