Removal of the product from the culture medium strongly enhances free fatty acid production by genetically engineered Synechococcus elongatus.

ABSTRACT:

Background

Cyanobacterial mutants engineered for production of free fatty acids (FFAs) secrete the products to the medium and hence are thought to be useful for biofuel production. The dAS1T mutant constructed from Synechococcus elongatus PCC 7942 has indeed a large capacity of FFA production, which is comparable to that of triacylglycerol production in green algae, but the yield of secreted FFAs is low because the cells accumulate most of the FFAs intracellularly and eventually die of their toxicity. To increase the FFA productivity, enhancement of FFA secretion is required.

Results

Growth of dAS1T cells but not WT cells was inhibited in a liquid medium supplemented with 0.13 g L^-1 of palmitic acid. This suggested that when FFA accumulates in the medium, i

SUBMITTER: Kato A 

PROVIDER: S-EPMC5452621 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature