A comparative approach to recombinantly produce the plant enzyme horseradish peroxidase in Escherichia coli.

ABSTRACT: Horseradish peroxidase (HRP) is used in various biotechnological and medical applications. Since its isolation from plant provides several disadvantages, the bacterium Escherichia coli was tested as recombinant expression host in former studies. However, neither production from refolded inclusion bodies nor active enzyme expression in the periplasm exceeded final titres of 10mg per litre cultivation broth. Thus, the traditional way of production of HRP from plant still prevails. In this study, we revisited the recombinant production of HRP in E. coli and investigated and compared both strategies, (a) the production of HRP as inclusion bodies (IBs) and subsequent refolding and (b) the production of active HRP in the periplasm. In fact, we were able to produce HRP in E. coli either way. We o

SUBMITTER: Gundinger T 

PROVIDER: S-EPMC5453243 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature