Caveolin1 protects against diet induced hepatic lipid accumulation in mice.

ABSTRACT:

Background and aim

Caveolin1 (CAV1) is involved in lipid homeostasis and endocytosis, but little is known about the significance of CAV1 in the pathogenesis and development of nonalcoholic fatty liver disease (NAFLD). This study aimed to determine the role of CAV1 in NAFLD.

Methods

Expression of CAV1 in the in vitro and in vivo models of NAFLD was analyzed. The effects of CAV1 knockdown or overexpression on free fatty acid (FFA)-induced lipid accumulation in L02 cells and AML12 cells were determined. CAV1 knockout (CAV1-KO) mice and their wild-type (WT) littermates were subjected to a high fat diet (HFD) for 4 weeks, and the functional consequences of losing the CAV1 gene and its subsequent molecular mechanisms were also examined.

Results

Noticeably, CAV1 expression w

SUBMITTER: Li M 

PROVIDER: S-EPMC5453590 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature