Detection of a microbial metabolite by STING regulates inflammasome activation in response to Chlamydia trachomatis infection.

ABSTRACT: The innate immune system is a critical component of host defence against microbial pathogens, but effective responses require an ability to distinguish between infectious and non-infectious insult to prevent inappropriate inflammation. Using the important obligate intracellular human pathogen Chlamydia trachomatis; an organism that causes significant immunopathology, we sought to determine critical host and pathogen factors that contribute to the induction of inflammasome activation. We assayed inflammasome activation by immunoblotting and ELISA to detect IL-1β processing and LDH release to determine pyroptosis. Using primary murine bone marrow derived macrophages or human monocyte derived dendritic cells, infected with live or attenuated Chlamydia trachomatis we report that the live organ

SUBMITTER: Webster SJ 

PROVIDER: S-EPMC5453623 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature