MicroRNA-150 modulates intracellular Ca ²⁺ levels in naive CD8⁺ T cells by targeting TMEM20.

ABSTRACT: Regulation of intracellular Ca²⁺ signaling is a major determinant of CD8⁺ T cell responsiveness, but the mechanisms underlying this regulation of Ca²⁺ levels, especially in naïve CD8⁺ T cells, are not fully defined. Here, we showed that microRNA-150 (miR-150) controls intracellular Ca²⁺ levels in naïve CD8⁺ T cells required for activation by suppressing TMEM20, a negative regulator of Ca²⁺ extrusion. miR-150 deficiency increased TMEM20 expression, which resulted in increased intracellular Ca²⁺ levels in naïve CD8⁺ T cells. The subsequent increase in Ca²⁺ levels induced expression of anergy-inducing genes, such as Cbl-b, Egr2, and p27, through activation of NFAT1, as well as reduced

SUBMITTER: Kim TD 

PROVIDER: S-EPMC5453935 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature