Quantitation and prediction of sorptive losses during toxicity testing of polycyclic aromatic hydrocarbon (PAH) and nitrated PAH (NPAH) using polystyrene 96-well plates.

ABSTRACT: Developing zebrafish are increasingly being used for rapid assessments of chemical toxicity, and these assays are frequently conducted in multi-well plastic plates. This study investigated the sorptive behavior of polycyclic aromatic hydrocarbons (PAHs) and nitrated PAHs (NPAHs) to uncoated 96-well polystyrene plates typically used for zebrafish (Danio rerio) testing. We measured the percent sorption in the presence and absence of zebrafish embryos, at two exposure concentrations, as well as using two different procedures (addition of embryos to polystyrene plates either before analyte addition, or allowing 24h of equilibrium between analyte addition and embryo addition to the polystyrene plates). Following exposure, the plates were extracted with hexane and analyzed using gas chromatograp

SUBMITTER: Chlebowski AC 

PROVIDER: S-EPMC5454028 | biostudies-literature | 2016 Sep - Oct

REPOSITORIES: biostudies-literature