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Complete Genome Sequence of Escherichia coli BLR(DE3), a recA-Deficient Derivative of E. coli BL21(DE3).

ABSTRACT: Escherichia coli BLR(DE3) is a commercially available recA-deficient derivative of BL21(DE3), one of the most widely used strains for recombinant protein expression. Here, we present the full-genome sequence of BLR(DE3) and highlight additional differences with its parent strain BL21(DE3) which were previously unreported but may affect its physiology.

SUBMITTER: Goffin P 

PROVIDER: S-EPMC5454202 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Complete Genome Sequence of <i>Escherichia coli</i> BLR(DE3), a <i>recA</i>-Deficient Derivative of <i>E. coli</i> BL21(DE3).

Goffin Philippe P   Dehottay Philippe P  

Genome announcements 20170601 22

<i>Escherichia coli</i> BLR(DE3) is a commercially available <i>recA</i>-deficient derivative of BL21(DE3), one of the most widely used strains for recombinant protein expression. Here, we present the full-genome sequence of BLR(DE3) and highlight additional differences with its parent strain BL21(DE3) which were previously unreported but may affect its physiology. ...[more]

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