Detecting tissue-specific regulation of alternative splicing as a qualitative change in microarray data.

ABSTRACT: Alternative splicing has recently emerged as a major mechanism of regulation in the human genome, occurring in perhaps 40-60% of human genes. Thus, microarray studies of functional regulation could, in principle, be extended to detect not only the changes in the overall expression of a gene, but also changes in its splicing pattern between different tissues. However, since changes in the total expression of a gene and changes in its alternative splicing can be mixed in complex ways among a set of samples, separating these effects can be difficult, and is essential for their accurate assessment. We present a simple and general approach for distinguishing changes in alternative splicing from changes in expression, based on detecting systematic anti-correlation between the log-ratios of two d

SUBMITTER: Le K 

PROVIDER: S-EPMC545471 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature