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Identification and Expression Profiling of the Auxin Response Factors in Dendrobium officinale under Abiotic Stresses.


ABSTRACT: Auxin response factor (ARF) proteins play roles in plant responses to diverse environmental stresses by binding specifically to the auxin response element in the promoters of target genes. Using our latest public Dendrobium transcriptomes, a comprehensive characterization and analysis of 14 DnARF genes were performed. Three selected DnARFs, including DnARF1, DnARF4, and DnARF6, were confirmed to be nuclear proteins according to their transient expression in epidermal cells of Nicotiana benthamiana leaves. Furthermore, the transcription activation abilities of DnARF1, DnARF4, and DnARF6 were tested in a yeast system. Our data showed that DnARF6 is a transcriptional activator in Dendrobium officinale. To unco

SUBMITTER: Chen Z 

PROVIDER: S-EPMC5454840 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature