Expression of Root Genes in Arabidopsis Seedlings Grown by Standard and Improved Growing Methods.

ABSTRACT: Roots of Arabidopsis thaliana seedlings grown in the laboratory using the traditional plant-growing culture system (TPG) were covered to maintain them in darkness. This new method is based on a dark chamber and is named the improved plant-growing method (IPG). We measured the light conditions in dark chambers, and found that the highest light intensity was dramatically reduced deeper in the dark chamber. In the bottom and side parts of dark chambers, roots were almost completely shaded. Using the high-throughput RNA sequencing method on the whole RNA extraction from roots, we compared the global gene expression levels in roots of seedlings from these two conditions and identified 141 differently expressed genes (DEGs) between them. According to the KEGG (Kyoto Encyclopedia of Genes

SUBMITTER: Qu Y 

PROVIDER: S-EPMC5454864 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature