A computational and experimental approach to validating annotations and gene predictions in the Drosophila melanogaster genome.

ABSTRACT: Five years after the completion of the sequence of the Drosophila melanogaster genome, the number of protein-coding genes it contains remains a matter of debate; the number of computational gene predictions greatly exceeds the number of validated gene annotations. We have assembled a collection of >10,000 gene predictions that do not overlap existing gene annotations and have developed a process for their validation that allows us to efficiently prioritize and experimentally validate predictions from various sources by sequencing RT-PCR products to confirm gene structures. Our data provide experimental evidence for 122 protein-coding genes. Our analyses suggest that the entire collection of predictions contains only approximately 700 additional protein-coding genes. Although we cannot rule

SUBMITTER: Yandell M 

PROVIDER: S-EPMC545494 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature