Comparison of molecular serotyping approaches of Streptococcus agalactiae from genomic sequences.

ABSTRACT:

Background

Group B streptococcus (GBS) capsular polysaccharide is one of the major virulence factors underlying invasive GBS disease and a component of forthcoming vaccines. Serotype classification of GBS is based on the capsule polysaccharide of which ten variants are known to exist (Ia, Ib, II-IX). Current methods for GBS serotype assignment rely on latex agglutination or PCR while more recently a whole genome sequencing method was reported. In this study, three distinct algorithms for serotype assignment from genomic data were assessed using a panel of 790 clinical isolates.

Methods

The first approach utilised the entire capsular locus coupled with a mapping methodology. The second approach continues from the first and utilised a SNP-based methodology across the conserved

SUBMITTER: Kapatai G 

PROVIDER: S-EPMC5455115 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature