A novel near-infrared fluorescent probe for sensitive detection of β-galactosidase in living cells.

ABSTRACT: A novel near-infrared fluorescent probe for β-galactosidase has been developed based on a hemicyanine skeleton, which is conjugated with a d-galactose residue via a glycosidic bond. The probe serves as a substrate of β-galactosidase and displays rapid and sensitive turn-on fluorescent responses to β-galactosidase in aqueous solution. A 12.8-fold enhancement of fluorescence intensity at 703 nm was observed after incubation of 10 nM of β-galactosidase with 5 μM probe for 10 min. The probe can sensitively detect as little as 0.1 nM of β-galactosidase and shows linear responses to the enzyme concentration below 1.4 nM. The kinetic study showed that the probe has high binding affinity to β-galactosidase with K_m = 3.6 μM. The probe was used to detect β-galactosidase in living cells by

SUBMITTER: Zhang J 

PROVIDER: S-EPMC5455349 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature