A comprehensive transcript index of the human genome generated using microarrays and computational approaches.

ABSTRACT:

Background

Computational and microarray-based experimental approaches were used to generate a comprehensive transcript index for the human genome. Oligonucleotide probes designed from approximately 50,000 known and predicted transcript sequences from the human genome were used to survey transcription from a diverse set of 60 tissues and cell lines using ink-jet microarrays. Further, expression activity over at least six conditions was more generally assessed using genomic tiling arrays consisting of probes tiled through a repeat-masked version of the genomic sequence making up chromosomes 20 and 22.

Results

The combination of microarray data with extensive genome annotations resulted in a set of 28,456 experimentally supported transcripts. This set of high-confidence transcr

SUBMITTER: Schadt EE 

PROVIDER: S-EPMC545593 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature