A reverse genetic screen in Drosophila using a deletion-inducing mutagen.

ABSTRACT: We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragment-length polymorphisms on standard DNA sequencers is implemented. As the spectrum of HMPA-induced mutations is similar in a variety of organisms, it should be possible to transfer this mutagenesis and detection procedure to other model systems.

SUBMITTER: Nairz K 

PROVIDER: S-EPMC545603 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature