A three-component regulatory system regulates biofilm maturation and type III secretion in Pseudomonas aeruginosa.

ABSTRACT: Biofilms are structured communities found associated with a wide range of surfaces. Here we report the identification of a three-component regulatory system required for biofilm maturation by Pseudomonas aeruginosa strain PA14. A transposon mutation that altered biofilm formation in a 96-well dish assay originally defined this locus, which is comprised of genes for a putative sensor histidine kinase and two response regulators and has been designated sadARS. Nonpolar mutations in any of the sadARS genes result in biofilms with an altered mature structure but do not confer defects in growth or early biofilm formation, swimming, or twitching motility. After 2 days of growth under flowing conditions, biofilms formed by the mutants are indistinguishable from those formed by the wild-type (WT)

SUBMITTER: Kuchma SL 

PROVIDER: S-EPMC545632 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature