Changes in the leaf proteome profile of Withania somnifera (L.) Dunal in response to Alternaria alternata infection.

ABSTRACT: Withania somnifera is a high value medicinal plant which is used against large number of ailments. The medicinal properties of the plant attributes to a wide array of important secondary metabolites. The plant is predominantly infected with leaf spot pathogen Alternaria alternata, which leads to substantial biodeterioration of pharmaceutically important metabolites. To develop an effective strategy to combat this disease, proteomics based approach could be useful. Hence, in the present study, three different protein extraction methods tris-buffer based, phenol based and trichloroacetic acid-acetone (TCA-acetone) based method were comparatively evaluated for two-dimensional electrophoresis (2-DE) analysis of W. somnifera. TCA-acetone method was found to be most effective and was further use

SUBMITTER: Singh V 

PROVIDER: S-EPMC5456394 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature