Nucleolus association of chromosomal domains is largely maintained in cellular senescence despite massive nuclear reorganisation.

ABSTRACT: Mammalian chromosomes are organized in structural and functional domains of 0.1-10 Mb, which are characterized by high self-association frequencies in the nuclear space and different contact probabilities with nuclear sub-compartments. They exhibit distinct chromatin modification patterns, gene expression levels and replication timing. Recently, nucleolus-associated chromosomal domains (NADs) have been discovered, yet their precise genomic organization and dynamics are still largely unknown. Here, we use nucleolus genomics and single-cell experiments to address these questions in human embryonic fibroblasts during replicative senescence. Genome-wide mapping reveals 1,646 NADs in proliferating cells, which cover about 38% of the annotated human genome. They are mainly heterochromatic and co

SUBMITTER: Dillinger S 

PROVIDER: S-EPMC5456395 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature