A small molecule screen identifies in vivo modulators of peripheral nerve regeneration in zebrafish.

ABSTRACT: Adult vertebrates have retained the ability to regenerate peripheral nerves after injury, although regeneration is frequently incomplete, often leading to functional impairments. Small molecule screens using whole organisms have high potential to identify biologically relevant targets, yet currently available assays for in vivo peripheral nerve regeneration are either very laborious and/or require complex technology. Here we take advantage of the optical transparency of larval zebrafish to develop a simple and fast pectoral fin removal assay that measures peripheral nerve regeneration in vivo. Twenty-four hours after fin amputation we observe robust and stereotyped nerve regrowth at the fin base. Similar to laser mediated nerve transection, nerve regrowth after fin amputation requires Schw

SUBMITTER: Bremer J 

PROVIDER: S-EPMC5456414 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature