Fickian-Based Empirical Approach for Diffusivity Determination in Hollow Alginate-Based Microfibers Using 2D Fluorescence Microscopy and Comparison with Theoretical Predictions.

ABSTRACT: Hollow alginate microfibers (od = 1.3 mm, id = 0.9 mm, th = 400 µm, L = 3.5 cm) comprised of 2% (w/v) medium molecular weight alginate cross-linked with 0.9 M CaCl₂ were fabricated to model outward diffusion capture by 2D fluorescent microscopy. A two-fold comparison of diffusivity determination based on real-time diffusion of Fluorescein isothiocyanate molecular weight (FITC MW) markers was conducted using a proposed Fickian-based approach in conjunction with a previously established numerical model developed based on spectrophotometric data. Computed empirical/numerical (D_empiricial/D_numerical) diffusivities characterized by small standard deviations for the 4-, 70- and 500-kDa markers expressed in m²/s are (1.

SUBMITTER: Mobed-Miremadi M 

PROVIDER: S-EPMC5456451 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature