Capturing the radical ion-pair intermediate in DNA guanine oxidation.

ABSTRACT: Although the radical ion pair has been frequently invoked as a key intermediate in DNA oxidative damage reactions and photoinduced electron transfer processes, the unambiguous detection and characterization of this species remain formidable and unresolved due to its extremely unstable nature and low concentration. We use the strategy that, at cryogenic temperatures, the transient species could be sufficiently stabilized to be detectable spectroscopically. By coupling the two techniques (the cryogenic stabilization and the time-resolved laser flash photolysis spectroscopy) together, we are able to capture the ion-pair transient G^+•⋯Cl^- in the chlorine radical-initiated DNA guanine (G) oxidation reaction, and provide direct evidence to ascertain the intricate type of ad

SUBMITTER: Jie J 

PROVIDER: S-EPMC5457143 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature