Polycomb Repressive Complex 2-Mediated Chromatin Repression Guides Effector CD8⁺ T Cell Terminal Differentiation and Loss of Multipotency.

ABSTRACT: Understanding immunological memory formation depends on elucidating how multipotent memory precursor (MP) cells maintain developmental plasticity and longevity to provide long-term immunity while other effector cells develop into terminally differentiated effector (TE) cells with limited survival. Profiling active (H3K27ac) and repressed (H3K27me3) chromatin in naive, MP, and TE CD8⁺ T cells during viral infection revealed increased H3K27me3 deposition at numerous pro-memory and pro-survival genes in TE relative to MP cells, indicative of fate restriction, but permissive chromatin at both pro-memory and pro-effector genes in MP cells, indicative of multipotency. Polycomb repressive complex 2 deficiency impaired clonal expansion and TE cell differentiation, but minimally impacted

SUBMITTER: Gray SM 

PROVIDER: S-EPMC5457165 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature