Bone Marrow Cell Trafficking Analyzed by ⁸⁹Zr-oxine Positron Emission Tomography in a Murine Transplantation Model.

ABSTRACT: Purpose: The success of hematopoietic stem cell transplantation (HSCT) depends on donor cell homing to the bone marrow. However, there is no reliable method of noninvasively monitoring the kinetics and distribution of transferred cells. Using zirconium-89 (⁸⁹Zr)-oxine cell labeling combined with PET imaging, we sought to visualize and quantify donor cell homing in a mouse bone marrow transplantation model.Experimental Design: The effect of ⁸⁹Zr-oxine labeling on bone marrow cell viability and differentiation was evaluated in vitro⁸⁹Zr-labeled bone marrow cells (2 × 10⁷ cells, 16.6 kBq/10⁶ cells) were transferred intravenously, and serial microPET images were obtained (n = 5). The effect of a CXCR4 inhibitor,

SUBMITTER: Asiedu KO 

PROVIDER: S-EPMC5457332 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature