Activating transcription factor 3 promotes embryo attachment via up-regulation of leukemia inhibitory factor in vitro.

ABSTRACT:

Background

A receptive endometrium is essential for maternal-embryonic molecular communication during implantation. However, the specific molecular regulatory mechanisms of the endometrial capacity remain poorly understood. Here, we examined activating transcription factor 3 (ATF3) expression in human endometria and the functional effect of ATF3 on embryo attachment in vitro.

Methods

Immunohistochemistry (IHC) was used to assess the ATF3 expression patterns in human endometria. Quantitative real-time PCR (qRT-PCR), western blotting and immunofluorescence (IF) studies were applied to explore ATF3 expression in Ishikawa cells upon estrogen (E₂) and medroxyprogesterone acetate (MPA) treatment. qRT-PCR and western blotting were performed to inspect LIF (leukemia inhib

SUBMITTER: Cheng X 

PROVIDER: S-EPMC5457579 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature