Novel APC gene mutations associated with protein alteration in diffuse type gastric cancer.

ABSTRACT:

Background

The role of adenomatous polyposis coli (APC) gene in mitosis might be critical for regulation of genomic stability and chromosome segregation. APC gene mutations have been associated to have a role in colon cancer and since gastric and colon tumors share some common genetic lesions, it is relevant to investigate the role of APC tumor suppressor gene in gastric cancer.

Methods

We investigated for somatic mutations in the Exons 14 and 15 of APC gene from 40 diffuse type gastric cancersamples. Rabbit polyclonal anti-APC antibody was used, which detects the wild-type APC protein and was recommended for detection of the respective protein in human tissues. Cell cycle analysis was done from tumor and adjacent normal tissue.

Results

APC immunoreactivity showed pos

SUBMITTER: Ghatak S 

PROVIDER: S-EPMC5457612 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature