Transcriptome analysis of haploid male gametophyte development in Arabidopsis.

ABSTRACT:

Background

The haploid male gametophyte generation of flowering plants consists of two- or three-celled pollen grains. This functional specialization is thought to be a key factor in the evolutionary success of flowering plants. Moreover, pollen ontogeny is also an attractive model in which to dissect cellular networks that control cell growth, asymmetric cell division and cellular differentiation. Our objective, and an essential step towards the detailed understanding of these processes, was to comprehensively define the male haploid transcriptome throughout development.

Results

We have developed staged spore isolation procedures for Arabidopsis and used Affymetrix ATH1 genome arrays to identify a total of 13,977 male gametophyte-expressed mRNAs, 9.7% of which were male-gam

SUBMITTER: Honys D 

PROVIDER: S-EPMC545776 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature