Crystal structure of the multiple antibiotic resistance regulator MarR from Clostridium difficile.

ABSTRACT: Regulators of multiple antibiotic resistance (MarRs) are key players against toxins in prokaryotes. MarR homologues have been identified in many bacterial and archaeal species which pose daunting antibiotic resistance issues that threaten public health. The continuous prevalence of Clostridium difficile infection (CDI) throughout the world is associated with the abuse of antibiotics, and antibiotic treatments of CDI have limited effect. In the genome of C. difficile strain 630, the marR gene (ID 4913953) encodes a MarR protein. Here, MarR from C. difficile (MarR_C.difficile) was subcloned and crystallized for the first time. MarR_C.difficile was successfully expressed in Escherichia coli in a soluble form and was purified to near-homogeneity (>95%) by a two-step purific

SUBMITTER: Peng JW 

PROVIDER: S-EPMC5458394 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature