Membrane Ballooning in Aggregated Platelets is Synchronised and Mediates a Surge in Microvesiculation.

ABSTRACT: Human platelet transformation into balloons is part of the haemostatic response and thrombus architecture. Here we reveal that in aggregates of platelets in plasma, ballooning in multiple platelets occurs in a synchronised manner. This suggests a mechanism of coordination between cells, previously unrecognised. We aimed to understand this mechanism, and how it may contribute to thrombus development. Using spinning-disc confocal microscopy we visualised membrane ballooning in human platelet aggregates adherent to collagen-coated surfaces. Within an aggregate, multiple platelets undergo ballooning in a synchronised fashion, dependent upon extracellular calcium, in a manner that followed peak cytosolic calcium levels in the aggregate. Synchrony was observed in platelets within but not between

SUBMITTER: Agbani EO 

PROVIDER: S-EPMC5459805 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature