Clonally stable Vκ allelic choice instructs Igκ repertoire.

ABSTRACT: Although much has been done to understand how rearrangement of the Igκ locus is regulated during B-cell development, little is known about the way the variable (V) segments themselves are selected. Here we show, using B6/Cast hybrid pre-B-cell clones, that a limited number of V segments on each allele is stochastically activated as characterized by the appearance of non-coding RNA and histone modifications. The activation states are clonally distinct, stable across cell division and developmentally important in directing the Ig repertoire upon differentiation. Using a new approach of allelic ATAC-seq, we demonstrate that the Igκ V alleles have differential chromatin accessibility, which may serve as the underlying basis of clonal maintenance at this locus, as well as other instances of mon

SUBMITTER: Levin-Klein R 

PROVIDER: S-EPMC5459994 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature