A semi high-throughput method for screening small bispecific antibodies with high cytotoxicity.

ABSTRACT: Small bispecific antibodies that induce T-cell-mediated cytotoxicity have the potential to damage late-stage tumor masses to a clinically relevant degree, but their cytotoxicity is critically dependent on their structural and functional properties. Here, we constructed an optimized procedure for identifying highly cytotoxic antibodies from a variety of the T-cell-recruiting antibodies engineered from a series of antibodies against cancer antigens of epidermal growth factor receptor family and T-cell receptors. By developing and applying a set of rapid operations for expression vector construction and protein preparation, we screened the cytotoxicity of 104 small antibodies with diabody format and identified some with 10³-times higher cytotoxicity than that of previously reported

SUBMITTER: Sugiyama A 

PROVIDER: S-EPMC5460266 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature