Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells.

ABSTRACT: Human pluripotent stem cells are a potentially powerful cellular resource for application in regenerative medicine. Because such applications require large numbers of human pluripotent stem cell-derived cells, a scalable culture system of human pluripotent stem cell needs to be developed. Several suspension culture systems for human pluripotent stem cell expansion exist; however, it is difficult to control the thickness of cell aggregations in these systems, leading to increased cell death likely caused by limited diffusion of gases and nutrients into the aggregations. Here, we describe a scalable culture system using the cell fiber technology for the expansion of human induced pluripotent stem (iPS) cells. The cells were encapsulated and cultured within the core region of core-shell hydro

SUBMITTER: Ikeda K 

PROVIDER: S-EPMC5460280 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature