Molecular characterization of breast cancer cell lines through multiple omic approaches.

ABSTRACT:

Background

Breast cancer cell lines are frequently used as model systems to study the cellular properties and biology of breast cancer. Our objective was to characterize a large, commonly employed panel of breast cancer cell lines obtained from the American Type Culture Collection (ATCC 30-4500 K) to enable researchers to make more informed decisions in selecting cell lines for specific studies. Information about these cell lines was obtained from a wide variety of sources. In addition, new information about cellular pathways that are activated within each cell line was generated.

Methods

We determined key protein expression data using immunoblot analyses. In addition, two analyses on serum-starved cells were carried out to identify cellular proteins and pathways that are ac

SUBMITTER: Smith SE 

PROVIDER: S-EPMC5460504 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature