Lateral cortical Cdca7 expression levels are regulated by Pax6 and influence the production of intermediate progenitors.

ABSTRACT:

Background

We studied whether regulation of Cdca7 (Cell division cycle associated 7) expression by transcription factor Pax6 contributes to Pax6's cellular actions during corticogenesis. The function of Cdca7 in mediating Pax6's effects during corticogenesis has not been explored. Pax6 is expressed by radial glial progenitors in the ventricular zone of the embryonic cortical neuroepithelium, where it is required for the development of a normal complement of Tbr2-expressing intermediate progenitor cells in the subventricular zone. Pax6's expression levels are graded across the ventricular zone, with highest levels laterally where Tbr2-expressing progenitors are generated in greatest numbers at early stages of corticogenesis.

Methods

We used in situ hybridization and immunohis

SUBMITTER: Huang YT 

PROVIDER: S-EPMC5460507 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature