A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples.

ABSTRACT:

Background

RNA-sequencing (RNA-seq) has emerged as one of the most sensitive tool for gene expression analysis. Among the library preparation methods available, the standard poly(A) + enrichment provides a comprehensive, detailed, and accurate view of polyadenylated RNAs. However, on samples of suboptimal quality ribosomal RNA depletion and exon capture methods have recently been reported as better alternatives.

Methods

We compared for the first time three commercial Illumina library preparation kits (TruSeq Stranded mRNA, TruSeq Ribo-Zero rRNA Removal, and TruSeq RNA Access) as representatives of these three different approaches using well-established human reference RNA samples from the MAQC/SEQC consortium on a wide range of input amounts (from 100 ng down to 1 ng) and de

SUBMITTER: Schuierer S 

PROVIDER: S-EPMC5460543 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature