Multilocus sequence typing of Cryptosporidium hominis from northern India.

ABSTRACT:

Background & objectives

Human cryptosporidiosis is endemic worldwide, and at least eight species have been reported in humans; the most common being Cryptosporidium hominis and C. parvum. Detailed understanding of the epidemiology of Cryptosporidium is increasingly facilitated using standardized universal technique for species differentiation and subtyping. In this study micro- and minisatellite targets in chromosome 6 were used to assess genetic diversity of C. hominis by sequence length polymorphisms along with single nucleotide polymorphisms (SNPs).

Methods

A total of 84 Cryptosporidium positive stool specimens were subjected to speciation and genotyping using small subunit (SSU) ribosomal RNA (rRNA) as the target gene. Genetic heterogeneity amongst C. hominis isolates wa

SUBMITTER: Yadav P 

PROVIDER: S-EPMC5460555 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature