Congressing kinetochores progressively load Ska complexes to prevent force-dependent detachment.

ABSTRACT: Kinetochores mediate chromosome congression by either sliding along the lattice of spindle microtubules or forming end-on attachments to their depolymerizing plus-ends. By following the fates of individual kinetochores as they congress in live cells, we reveal that the Ska complex is required for a distinct substep of the depolymerization-coupled pulling mechanism. Ska depletion increases the frequency of naturally occurring, force-dependent P kinetochore detachment events, while being dispensable for the initial biorientation and movement of chromosomes. In unperturbed cells, these release events are followed by reattachment and successful congression, whereas in Ska-depleted cells, detached kinetochores remain in a futile reattachment/detachment cycle that prevents congression. We furthe

SUBMITTER: Auckland P 

PROVIDER: S-EPMC5461014 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature