Systemic innate immune activation in food protein-induced enterocolitis syndrome.

ABSTRACT:

Background

Food protein-induced enterocolitis syndrome (FPIES) is a non-IgE-mediated food allergy of infancy whose pathophysiology is poorly understood.

Objectives

We set out to identify and phenotype allergen-responsive cells in peripheral blood of a cohort of subjects undergoing supervised food challenge for FPIES.

Methods

We profiled antigen-responsive cells in PBMCs by flow cytometry, and examined cells in whole blood obtained before and after challenge by CyTOF mass cytometry and RNAseq.

Results

Using a CD154-based detection approach, we observed that milk, soy, or rice-responsive T cells, and TNF-α-producing CD154⁺ T cells, were significantly lower in those with outgrown FPIES compared with those with active FPIES. However, levels were within t

SUBMITTER: Goswami R 

PROVIDER: S-EPMC5461215 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature