Down-regulation of Irf8 by Lyz2-cre/loxP accelerates osteoclast differentiation in vitro.

ABSTRACT: Interferon regulatory factor 8 (Irf8) is a transcription factor that negatively regulates osteoclast differentiation and Irf8 global knockout (Irf8 ^-/-) mice have been shown to have reduced bone volume resulting from increased osteoclast numbers. However, detailed analysis of the functions of Irf8 in osteoclast precursors with a monocyte/macrophage linage is difficult, because the population and properties of hematopoietic cells in Irf8 ^-/- mice are severely altered. Therefore, to clearly elucidate the functions of Irf8 during osteoclastogenesis, we established myeloid cell-specific Irf8 conditional knockout (Irf8 ^fl/fl ;Lyz2 ^cre/+) mice. We found that trabecular bone volume in the Irf8 ^fl/fl ;Lyz2 ^cre/+ mice was not signifi

SUBMITTER: Saito E 

PROVIDER: S-EPMC5461233 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature