Rationally Designed Influenza Virus Vaccines That Are Antigenically Stable during Growth in Eggs.

ABSTRACT: Influenza virus vaccine production is currently limited by the ability to grow circulating human strains in chicken eggs or in cell culture. To facilitate cost-effective growth, vaccine strains are serially passaged under production conditions, which frequently results in mutations of the major antigenic protein, the viral hemagglutinin (HA). Human vaccination with an antigenically drifted strain is known to contribute to poor vaccine efficacy. To address this problem, we developed a replication-competent influenza A virus (IAV) with an artificial genomic organization that allowed the incorporation of two independent and functional HA proteins with different growth requirements onto the same virion. Vaccination with these viruses induced protective immunity against both strains from which

SUBMITTER: Harding AT 

PROVIDER: S-EPMC5461409 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature