Functional Roles of Netrin-1 in Osteoblast Differentiation.

ABSTRACT:

Aim

Recent studies have demonstrated that netrin-1 plays a vital role in bone metabolism. Previous studies have shown that osteoblasts produce netrin-1 which affects osteoclast differentiation. However, the role of netrin-1 in osteoblast differentiation is not well understood. In this study, we explored the roles of netrin-1 in osteoblasts.

Materials and methods

Quantitative reverse-transcriptase polymerase chain reaction (qPCR), RNA interference for netrin receptors, the generation of netrin-1 plasmid, transfection of plasmids, and cell proliferation assay were performed.

Results

During osteoblast differentiation by ascorbic acid, netrin-1 expression was significantly decreased. Gene expression related with osteoblast differentiation was down-regulated by netrin-1 tr

SUBMITTER: Sato T 

PROVIDER: S-EPMC5461440 | biostudies-literature | 2017 May-Jun

REPOSITORIES: biostudies-literature