Typing and Characterization of Bacteria Using Bottom-up Tandem Mass Spectrometry Proteomics.

ABSTRACT: Methods for rapid and reliable microbial identification are essential in modern healthcare. The ability to detect and correctly identify pathogenic species and their resistance phenotype is necessary for accurate diagnosis and efficient treatment of infectious diseases. Bottom-up tandem mass spectrometry (MS) proteomics enables rapid characterization of large parts of the expressed genes of microorganisms. However, the generated data are highly fragmented, making downstream analyses complex. Here we present TCUP, a new computational method for typing and characterizing bacteria using proteomics data from bottom-up tandem MS. TCUP compares the generated protein sequence data to reference databases and automatically finds peptides suitable for characterization of taxonomic composition and id

SUBMITTER: Boulund F 

PROVIDER: S-EPMC5461537 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature