Analysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa.

ABSTRACT:

Background

Lobosphaera incisa (L. incisa) is an oleaginous microalga that stores triacylglycerol (TAG) rich in arachidonic acid in lipid bodies (LBs). This organelle is gaining attention in algal research, since evidence is accumulating that proteins attached to its surface fulfill important functions in TAG storage and metabolism.

Results

Here, the composition of the LB proteome in L incisa was investigated by comparing different cell fractions in a semiquantitative proteomics approach. After applying stringent filters to the proteomics data in order to remove contaminating proteins from the list of possible LB proteins (LBPs), heterologous expression of candidate proteins in tobacco pollen tubes, allowed us to confirm 3 true LBPs: A member of the algal Major Lipid Droplet

SUBMITTER: Siegler H 

PROVIDER: S-EPMC5461629 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature