Immune responses upon Campylobacter jejuni infection of secondary abiotic mice lacking nucleotide-oligomerization-domain-2.

ABSTRACT:

Background

Campylobacter jejuni infections are of rising importance worldwide. Given that innate immune receptors including nucleotide-oligomerization-domain-2 (Nod2) are essentially involved in combating enteropathogenic infections, we here surveyed the impact of Nod2 in murine campylobacteriosis.

Methods and results

In order to overcome physiological colonization resistance preventing from C. jejuni infection, we generated secondary abiotic Nod2^-/- and wildtype (WT) mice by broad-spectrum antibiotic treatment. Mice were then perorally infected with C. jejuni strain 81-176 on 2 consecutive days and could be stably colonized by the pathogen at high loads. Notably, Nod2 deficiency did not affect gastrointestinal colonization properties of C.

SUBMITTER: Bereswill S 

PROVIDER: S-EPMC5461728 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature