Effect of tryptase inhibition on joint inflammation: a pharmacological and lentivirus-mediated gene transfer study.

ABSTRACT:

Background

Increasing evidences indicate that an unbalance between tryptases and their endogenous inhibitors, leading to an increased proteolytic activity, is implicated in the pathophysiology of rheumatoid arthritis. The aim of the present study was to evaluate the impact of tryptase inhibition on experimental arthritis.

Methods

Analysis of gene expression and regulation in the mouse knee joint was performed by RT-qPCR and in situ hybridization. Arthritis was induced in male C57BL/6 mice with mBSA/IL-1β. Tryptase was inhibited by two approaches: a lentivirus-mediated heterologous expression of the human endogenous tryptase inhibitor, sperm-associated antigen 11B isoform C (hSPAG11B/C), or a chronic treatment with the synthetic tryptase inhibitor APC366. Several inflammatory

SUBMITTER: Denadai-Souza A 

PROVIDER: S-EPMC5461776 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature