Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell.

ABSTRACT:

Background

Current methods for accurate quantification of nucleic acids typically begin with a template preparation step in which DNA and/or RNA are freed of bound proteins and are then purified. Isolation of RNA is particularly challenging because this molecule is sensitive to elevated temperatures and is degraded by RNases, which therefore have to be immediately inactivated upon cell lysis. Many protocols for nucleic acids purification, reverse transcription of RNA and/or amplification of DNA require repeated transfers from tube to tube and other manipulations during which materials may be lost.

Results

This paper introduces a novel and highly reliable single-tube method for rapid cell lysis, followed by quantitative preparation and analysis of both RNA and/or DNA molecule

SUBMITTER: Hartshorn C 

PROVIDER: S-EPMC546192 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature