Gene expression profiling of tumours derived from rasV12/E1A-transformed mouse embryonic fibroblasts to identify genes required for tumour development.

ABSTRACT:

Background

In cancer, cellular transformation is followed by tumour development. Knowledge on the mechanisms of transformation, involving activation of proto-oncogenes and inactivation of tumour-suppressor genes has considerably improved whereas tumour development remains poorly understood. An interesting way of gaining information on tumour progression mechanisms would be to identify genes whose expression is altered during tumour formation. We used the Affymetrix-based DNA microarray technology to analyze gene expression profiles of tumours derived from rasV12/E1A-transformed mouse embryo fibroblasts in order to identify the genes that could be involved in tumour development.

Results

Among the 12,000 genes analyzed in this study, only 489 showed altered expression during t

SUBMITTER: Vasseur S 

PROVIDER: S-EPMC546195 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature